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mouse anti mklp1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse anti mklp1
    Mouse Anti Mklp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mklp1/MKLP-1+Antibody/pmc12686533-219-73-76
    Average 93 stars, based on 71 article reviews
    mouse anti mklp1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The secreted midbody remnants, MBsomes, as a new class of mRNA nanocarrier applicable to diverse medical fields
    Article Snippet: .. After blocking with 3% bovine serum albumin (BSA) for 2 hours, the samples were reacted with an Alexa Fluor 488-conjugated anti- MKLP1(unique marker of MBsome) antibody (Santa Cruz Biotechnology, Dallas, TX, USA; diluted 1:50 in PBS containing 3% BSA) for 24 hours. .. Subsequently, the cells were washed with 200 μl PBS, sealed with a fixative solution, and analyzed via confocal laser scanning microscopy (TCS SP5, Leica Microsystems, Wetzlar, Germany).

    Marker:

    Article Title: The secreted midbody remnants, MBsomes, as a new class of mRNA nanocarrier applicable to diverse medical fields
    Article Snippet: .. After blocking with 3% bovine serum albumin (BSA) for 2 hours, the samples were reacted with an Alexa Fluor 488-conjugated anti- MKLP1(unique marker of MBsome) antibody (Santa Cruz Biotechnology, Dallas, TX, USA; diluted 1:50 in PBS containing 3% BSA) for 24 hours. .. Subsequently, the cells were washed with 200 μl PBS, sealed with a fixative solution, and analyzed via confocal laser scanning microscopy (TCS SP5, Leica Microsystems, Wetzlar, Germany).

    Labeling:

    Article Title: Commitment to cytokinetic furrowing requires the coordinate activity of microtubules and Plk1
    Article Snippet: To reduce free aldehyde groups, fixation was followed by treatment with 0.5 mg/ml NaBH 4 in cytoskeleton buffer for 5 min. .. Cells were labeled with combinations of the following: phalloidin-Alexa 594 (Invitrogen, Carlsbad, CA), anti-α-tubulin (mouse monoclonal D1a clone1:1000; Sigma), anti-Aurora B (1:1000, Abcam, Cambridge, MA), anti-PRC1 (1:1,000; Santa Cruz Biotechnology-SCBT, Santa Cruz, CA), anti-MyoII (1:500; Covance, Princeton, NJ), anti-MKLP1 (1:1000, SCBT), anti-RhoA, (1:100; SCBT), or anti-anillin (gift of Dr. Christine Field, Harvard Medical School). .. The 2° antibodies were the species appropriate Alexa-488, Alexa-594, or Alexa-660 at 1:500 dilution (Invitrogen, Carlsbad, CA).

    other:

    Article Title: K29-Linked Ubiquitin Signaling Regulates Proteotoxic Stress Response and Cell Cycle
    Article Snippet: Other primary antibodies are diluted as followed: Anti AuroraB (Fisher,1:500); Anti α-Tubulin (Genscript, 1:50-1:100); Anti ɑ-Tubulin (proteintech, 1:200); Anti c-Myc (Genscript, 0.5 μg/mL); Anti EIF3B (Santa Cruz, 1:100); Anti INCENP (Santa Cruz,1:100), Anti MKLP1(Santa Cruz, 1:100); Anti Proteasome 20S core subunits (Fisher, 1:250); Anti K48-Ub (Abcam, 1:200); Anti VCP (Santa Cruz,1:200); Anti G3BP1 (proteintech,1:500); Anti pTBK1 (cell signaling,1:50); Anti PLK1 (Santa Cruz,1:50).



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    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of <t>MKLP1</t> (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
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    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of <t>MKLP1</t> (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
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    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of <t>MKLP1</t> (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
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    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of <t>MKLP1</t> (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
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    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of <t>MKLP1</t> (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
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    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of <t>MKLP1</t> (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
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    Image Search Results


    Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of MKLP1 (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).

    Journal: Journal of Cell Science

    Article Title: MOS is a key regulator of meiotic midbody assembly and prevents abnormal divisions in mouse eggs and their polar bodies

    doi: 10.1242/jcs.264411

    Figure Lengend Snippet: Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of MKLP1 (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).

    Article Snippet: The primary antibodies used were: rabbit anti-MKLP1 polyclonal antibody (Novus NBP2-56923; 1:100) and rabbit anti-PRC1 polyclonal antibody (Proteintech 15617-1-AP; 1:100).

    Techniques: Immunofluorescence